亮氨酸脱氢酶（EC 1.4.1.9），亮氨酸脱氢酶催化亮氨酸和酮异己酸之间的转化，临床上可用于亮氨酸检测及亮氨酸氨基肽酶活性检测。
Appearance White powder, lyophilized
Source Microorganism
Enzyme Commission Number EC 1.4.1.9
CAS Number 9082-71-7
Storage temperature -20℃
Specific activity ≥ 25U/mg protein
Unit definition One unit will convert one micromole of L-Leucine to α-Ketoisocaproate per minute at pH 10.5 at 37℃
PROPERTIES
Molecular weight 43 kDa (SDS-PAGE)
Isoelectric point 6.6
Optimum pH:above11.0(L-Leu→α-K I C),
8.5(α-K I C→ L-Leu )
Optimum temperature: 55-60℃(L-Leu →α-K I C)
above 60℃ (α-K I C→ L-Leu )
pH Stability 6.0~11.0 (25℃, 15hr)
Thermal stability < 55℃ (pH 7.0, 20min)
Molecular weight 43 kDa (SDS-PAGE)
Isoelectric point 6.6
Optimum pH:above11.0(L-Leu→α-K I C),
8.5(α-K I C→ L-Leu )
Optimum temperature: 55-60℃(L-Leu →α-K I C)
above 60℃ (α-K I C→ L-Leu )
pH Stability 6.0~11.0 (25℃, 15hr)
Thermal stability < 55℃ (pH 7.0, 20min)
矢车菊素3-O-葡萄糖苷
Ammonia 100ppm as N